crispra library Search Results


gene  (Addgene inc)
94
Addgene inc gene
Gene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crispra+library/pmc12618816-49-23-24?v=Addgene+inc
Average 94 stars, based on 1 article reviews
gene - by Bioz Stars, 2026-08
94/100 stars
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crispra library  (Addgene inc)
93
Addgene inc crispra library
A. Flow cytometry of hXEN expressed hematopoietic markers CD34 and CD43 upon exposure to hematopoietic cytokines and Activin A culture for 7 days. B. Generation of THP1 monocyte expressing dCas9-VP64 indicated by GFP. Fluorescence microscope showed GFP+ THP1 expressing dCas9-VP64. Scale bar = 100 um. C. CD71 erythroblast marker was up in <t>CRISPRa-pooled</t> <t>gRNA-induced</t> THP1 monocytes. Compared with VP64 that downregulated CD71, addition of pooled gRNA (MOI 0.25 and 1.0, respectively) rescued the CD71 level than WT. D. hXEN expressing dCAS9-VP64 and gRNAs. hXEN (human extraembryonic endoderm).
Crispra Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crispra+library/bio_rxiv__2025__11__07__687183-170-10-12?v=Addgene+inc
Average 93 stars, based on 1 article reviews
crispra library - by Bioz Stars, 2026-08
93/100 stars
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se realizó un screening de crispra con dos librerías  (Addgene inc)
92
Addgene inc se realizó un screening de crispra con dos librerías
A. Flow cytometry of hXEN expressed hematopoietic markers CD34 and CD43 upon exposure to hematopoietic cytokines and Activin A culture for 7 days. B. Generation of THP1 monocyte expressing dCas9-VP64 indicated by GFP. Fluorescence microscope showed GFP+ THP1 expressing dCas9-VP64. Scale bar = 100 um. C. CD71 erythroblast marker was up in <t>CRISPRa-pooled</t> <t>gRNA-induced</t> THP1 monocytes. Compared with VP64 that downregulated CD71, addition of pooled gRNA (MOI 0.25 and 1.0, respectively) rescued the CD71 level than WT. D. hXEN expressing dCAS9-VP64 and gRNAs. hXEN (human extraembryonic endoderm).
Se Realizó Un Screening De Crispra Con Dos Librerías, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crispra+library/10__24875_slash_sangre__m25000086-6164-1-16?v=Addgene+inc
Average 92 stars, based on 1 article reviews
se realizó un screening de crispra con dos librerías - by Bioz Stars, 2026-08
92/100 stars
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jonathan weissman  (Addgene inc)
92
Addgene inc jonathan weissman
A. Flow cytometry of hXEN expressed hematopoietic markers CD34 and CD43 upon exposure to hematopoietic cytokines and Activin A culture for 7 days. B. Generation of THP1 monocyte expressing dCas9-VP64 indicated by GFP. Fluorescence microscope showed GFP+ THP1 expressing dCas9-VP64. Scale bar = 100 um. C. CD71 erythroblast marker was up in <t>CRISPRa-pooled</t> <t>gRNA-induced</t> THP1 monocytes. Compared with VP64 that downregulated CD71, addition of pooled gRNA (MOI 0.25 and 1.0, respectively) rescued the CD71 level than WT. D. hXEN expressing dCAS9-VP64 and gRNAs. hXEN (human extraembryonic endoderm).
Jonathan Weissman, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crispra+library/pm37714156-782-7-9?v=Addgene+inc
Average 92 stars, based on 1 article reviews
jonathan weissman - by Bioz Stars, 2026-08
92/100 stars
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sgrna crispra library  (Broad Institute Inc)
90
Broad Institute Inc sgrna crispra library
( A ) THP-1 cells were stained for intracellular TNF-α and sorted into TNF-α + , TNF-α – , TNF-α hi , and TNF-α lo populations. Enrichment and depletion of sgRNAs in TNF-α subpopulations were compared with presorted and TNF-α – populations. ( B ) Log fold-change of sgRNAs targeting the TSS of TNFA reveal correlation with TNF-α levels, thus validating the <t>CRISPRa</t> screening potential. ( C – F ) Log fold-change of sgRNAs targeting lncRNAs that positively ( lnc-MAP3K11-1 , C ) or negatively ( lnc-IL5-1 , D ; SENCR , E ; lncTMEM154-4 , F ) correlate with levels of TNF-α production.
Sgrna Crispra Library, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crispra+library/pmc10967393-303-1-10?v=Broad+Institute+Inc
Average 90 stars, based on 1 article reviews
sgrna crispra library - by Bioz Stars, 2026-08
90/100 stars
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human crispr-pooltm sam crispra library  (Broad Institute Inc)
90
Broad Institute Inc human crispr-pooltm sam crispra library
( A ) THP-1 cells were stained for intracellular TNF-α and sorted into TNF-α + , TNF-α – , TNF-α hi , and TNF-α lo populations. Enrichment and depletion of sgRNAs in TNF-α subpopulations were compared with presorted and TNF-α – populations. ( B ) Log fold-change of sgRNAs targeting the TSS of TNFA reveal correlation with TNF-α levels, thus validating the <t>CRISPRa</t> screening potential. ( C – F ) Log fold-change of sgRNAs targeting lncRNAs that positively ( lnc-MAP3K11-1 , C ) or negatively ( lnc-IL5-1 , D ; SENCR , E ; lncTMEM154-4 , F ) correlate with levels of TNF-α production.
Human Crispr Pooltm Sam Crispra Library, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crispra+library/pmc10079833__41467_2023_37542_MOESM12_ESM-94-7-23?v=Broad+Institute+Inc
Average 90 stars, based on 1 article reviews
human crispr-pooltm sam crispra library - by Bioz Stars, 2026-08
90/100 stars
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hif2 subpool crispra sgrna library  (Broad Institute Inc)
90
Broad Institute Inc hif2 subpool crispra sgrna library
( A ) THP-1 cells were stained for intracellular TNF-α and sorted into TNF-α + , TNF-α – , TNF-α hi , and TNF-α lo populations. Enrichment and depletion of sgRNAs in TNF-α subpopulations were compared with presorted and TNF-α – populations. ( B ) Log fold-change of sgRNAs targeting the TSS of TNFA reveal correlation with TNF-α levels, thus validating the <t>CRISPRa</t> screening potential. ( C – F ) Log fold-change of sgRNAs targeting lncRNAs that positively ( lnc-MAP3K11-1 , C ) or negatively ( lnc-IL5-1 , D ; SENCR , E ; lncTMEM154-4 , F ) correlate with levels of TNF-α production.
Hif2 Subpool Crispra Sgrna Library, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crispra+library/10__1158_slash_2159___8290__cd___24___1378-162-1-19?v=Broad+Institute+Inc
Average 90 stars, based on 1 article reviews
hif2 subpool crispra sgrna library - by Bioz Stars, 2026-08
90/100 stars
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cell type enriched genes  (Human Protein Atlas)
86
Human Protein Atlas cell type enriched genes
( A ) THP-1 cells were stained for intracellular TNF-α and sorted into TNF-α + , TNF-α – , TNF-α hi , and TNF-α lo populations. Enrichment and depletion of sgRNAs in TNF-α subpopulations were compared with presorted and TNF-α – populations. ( B ) Log fold-change of sgRNAs targeting the TSS of TNFA reveal correlation with TNF-α levels, thus validating the <t>CRISPRa</t> screening potential. ( C – F ) Log fold-change of sgRNAs targeting lncRNAs that positively ( lnc-MAP3K11-1 , C ) or negatively ( lnc-IL5-1 , D ; SENCR , E ; lncTMEM154-4 , F ) correlate with levels of TNF-α production.
Cell Type Enriched Genes, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crispra+library/bio_rxiv__64898__2025__12__03__692130-272-10-17?v=Human+Protein+Atlas
Average 86 stars, based on 1 article reviews
cell type enriched genes - by Bioz Stars, 2026-08
86/100 stars
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mouse subpooled crispra-v2 libraries (pooled libraries #83998, #83999, #84000, #84001, #84002, #84003, #84004)  (Addgene inc)
N/A
Kinases, Phosphatases, and Drug Targets - gRNA pooled library†
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Image Search Results


A. Flow cytometry of hXEN expressed hematopoietic markers CD34 and CD43 upon exposure to hematopoietic cytokines and Activin A culture for 7 days. B. Generation of THP1 monocyte expressing dCas9-VP64 indicated by GFP. Fluorescence microscope showed GFP+ THP1 expressing dCas9-VP64. Scale bar = 100 um. C. CD71 erythroblast marker was up in CRISPRa-pooled gRNA-induced THP1 monocytes. Compared with VP64 that downregulated CD71, addition of pooled gRNA (MOI 0.25 and 1.0, respectively) rescued the CD71 level than WT. D. hXEN expressing dCAS9-VP64 and gRNAs. hXEN (human extraembryonic endoderm).

Journal: bioRxiv

Article Title: Blood originates in hypoblasts during embryonic development

doi: 10.1101/2025.11.07.687183

Figure Lengend Snippet: A. Flow cytometry of hXEN expressed hematopoietic markers CD34 and CD43 upon exposure to hematopoietic cytokines and Activin A culture for 7 days. B. Generation of THP1 monocyte expressing dCas9-VP64 indicated by GFP. Fluorescence microscope showed GFP+ THP1 expressing dCas9-VP64. Scale bar = 100 um. C. CD71 erythroblast marker was up in CRISPRa-pooled gRNA-induced THP1 monocytes. Compared with VP64 that downregulated CD71, addition of pooled gRNA (MOI 0.25 and 1.0, respectively) rescued the CD71 level than WT. D. hXEN expressing dCAS9-VP64 and gRNAs. hXEN (human extraembryonic endoderm).

Article Snippet: The gRNA sequences for 30 genes were selected from the CRISPRa Library (Addgene #60956), with 5 gRNAs for each gene.

Techniques: Flow Cytometry, Expressing, Fluorescence, Microscopy, Marker

( A ) THP-1 cells were stained for intracellular TNF-α and sorted into TNF-α + , TNF-α – , TNF-α hi , and TNF-α lo populations. Enrichment and depletion of sgRNAs in TNF-α subpopulations were compared with presorted and TNF-α – populations. ( B ) Log fold-change of sgRNAs targeting the TSS of TNFA reveal correlation with TNF-α levels, thus validating the CRISPRa screening potential. ( C – F ) Log fold-change of sgRNAs targeting lncRNAs that positively ( lnc-MAP3K11-1 , C ) or negatively ( lnc-IL5-1 , D ; SENCR , E ; lncTMEM154-4 , F ) correlate with levels of TNF-α production.

Journal: JCI Insight

Article Title: Gene coexpression networks reveal a broad role for lncRNAs in inflammatory bowel disease

doi: 10.1172/jci.insight.168988

Figure Lengend Snippet: ( A ) THP-1 cells were stained for intracellular TNF-α and sorted into TNF-α + , TNF-α – , TNF-α hi , and TNF-α lo populations. Enrichment and depletion of sgRNAs in TNF-α subpopulations were compared with presorted and TNF-α – populations. ( B ) Log fold-change of sgRNAs targeting the TSS of TNFA reveal correlation with TNF-α levels, thus validating the CRISPRa screening potential. ( C – F ) Log fold-change of sgRNAs targeting lncRNAs that positively ( lnc-MAP3K11-1 , C ) or negatively ( lnc-IL5-1 , D ; SENCR , E ; lncTMEM154-4 , F ) correlate with levels of TNF-α production.

Article Snippet: An sgRNA CRISPRa library was designed and synthesized by the Broad Institute.

Techniques: Staining